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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Flicr , a long noncoding RNA, modulates Foxp3 expression and autoimmunity
doi: 10.1073/pnas.1700946114
Figure Lengend Snippet: Flicr is expressed specifically in Tregs. Flicr expression in various contexts. (A and B) ImmGen mouse immunocyte (39) or GNF mouse organ microarray compendia (40) (dashed lines: background level). (C) During thymic Treg differentiation. (Left) ImmGen data. (Right) RNA-Seq analysis of CD4+ SP thymocytes sorted as shown; y-axis, transcripts per million (tpm). (D) Tissue Tregs. Each point is an individual mouse. Sp, spleen; Col, colonic lamina propria; Panc, pancreas; Adip. tissue, visceral adipose tissue; Muscle, injured muscle at 4 d postcardiotoxin injection. (E) After activation in vitro with anti-CD3/CD28 beads (42) (Left) or in vivo (41) (Right). Rest., resting CD44loCD62Lhi; Act., activated CD44hiCD62Llo Tregs; rpkm, reads per kb per million reads. (F) In vitro converted induced Tregs (TCR activation with IL-2 and TGF-β) (43). (G) Ex vivo Helios−RORγ+ colonic peripheral Tregs (44). (H) In Tconvs transduced with Foxp3 or control retrovectors (11). (I) In Treg-like cells of mice with Foxp3 inactivation by Gfp insertion (Foxp3ΔGfp) (45).
Article Snippet: Total CD4 T cells from spleen and subcutaneous lymph nodes were isolated by magnetic negative selection (Dynabeads Untouched Mouse CD4 T-Cell Kit, 11415D; Thermo Fisher Scientific) and activated in vitro in 200 µL of cRMPI for 72 h with anti-CD3/CD28 beads in a
Techniques: Expressing, Microarray, RNA Sequencing Assay, Injection, Activation Assay, In Vitro, In Vivo, Ex Vivo, Transduction